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KMID : 0615720120290010015
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Jouranl of Korean Acupuncture and Moxibustion society
2012 Volume.29 No. 1 p.15 ~ p.24
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Effects of Cinnamomi Cortex Pharmacopuncture on LPS-induced Inflammatory Response in Macrophage
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Kim Hyeong-Seok
Roh Jeong-Du
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Abstract
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Objectives: In recent years, many studies have been widely researching anti-inflammation effect of various medicinal plants. Cinnamomi Cortex was not enough in researching of the anti-inflammation. Moreover, there is no comparative study about extraction methods. Therefore, we investigated the inhibitory effects of Cinnamomi Cortex pharmacopuncture by EtOH and Hot water extraction on Nitric oxide(NO), Prostaglandin E2(PGE2) production, Cyclooxygenase( COX)-2, inducible NOS(iNOS) expression and extracellular signal regulate kinase(ERK)1/2 phosphorylation in lipopolysaccharide(LPS) induced RAW 264.7 macrophage cell.
Methods: Cinnamomi Cortex was extracted by EtOH and Hot water. RAW 264.7 macrophage cell viability was measured by MTT assay. Effect of Cinnamomi Cortex pharmacopuncture on NO and PGE2 production in LPS induced macrophages was accessed by Griess assay and enzyme-linked immunospecific assay(ELISA), respectively. Inhibition effect on COX-2, iNOS expression and ERK1/2 phosphorylation was examined by Immunoblotting assay.
Results: 1. Cytotoxic effect of Cinnamomi Cortex pharmacopuncture by Hot water extraction in RAW 264.7 macrophages was not appeared, except 3125 ㎍/㎖. And cytotoxic effect was not appeared in EtOH extraction method. 2. Cinnamomi Cortex pharmacopuncture by EtOH and Hot water extraction inhibited NO production in LPS induced macrophages significantly. 3. Cinnamomi Cortex pharmacopuncture by EtOH and Hot water extraction inhibited PGE2 production in LPS induced macrophages significantly. 4. Cinnamomi Cortex pharmacopuncture by EtOH and Hot water extraction inhibited COX-2, iNOS expression in LPS induced macrophages. Especially, it has been confirmed that COX-2, iNOS expression were effectively inhibited in Hot water extraction. 5. Cinnamomi Cortex pharmacopuncture by EtOH and Hot water extraction inhibited ERK1/2 phosphorylation in LPS induced macrophages. Especially, it has been confirmed that ERK1/2 phosphorylation was effectively inhibited in Hot water extraction.
Conclusions: According to the results, Cinnamomi Cortex pharmacopuncture suppresses NO, PGE2 production, COX-2, iNOS expression and ERK1/2 phosphorylation in LPS induced macrophages. It has a potential for treating various inflammatory diseases, and Hot water extraction method could be used more extensively than EtOH extraction method.
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KEYWORD
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anti-inflammatory, Cinnamomi Cortex, COX-2, ERK1/2, iNOS, Korean medicine, NO, PGE2, pharmacopuncture
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